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Product Description
Recombinant Mouse Platelet Derived Growth Factor-AA is a disulfide linked dimer comprised of 2 polypeptide chains of 126 amino acids each, with a dimeric molecular weight of 28.9 kDa. Belonging to a family of PDGFs including PDGF-AA, PDGF-BB and PDGF-AB, they play an important role as a mitogen for a number of cell types. Recombinant Mouse PDGF-AA produced in E.Coli is a non-glycosylated homodimer.
Biovision | 4483 | PDGF-AA murine recombinant DataSheet
Biomolecule/Target: PDGF-AA
Synonyms: Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AA, PDGF-1.
Alternates names: GSK, Glycogen synthase kinase-3 (GSK-3)
Taglines: A growth factor that regulates cell growth and division
NCBI Gene ID #: 2932
NCBI Gene Symbol: GSK3B
Gene Source: Human
Accession #: P49841
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 90%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): Activity of GSK-3 was determined by an in vitro kinase assay using 100 ng Tau as substrate and 1 µg of the recombinant GSK-3 in a 20 µl reaction containing 25 mM Tris-HCl, pH 7.5, 5 mM -glycerol phosphate, 12 mM MgCl, 2 mM DTT, 0.1 M Na3VO4, and 200 µM ATP. After 30 minutes incubation at 30°C, phosphorylation of Tau by the recombinant GSK-3 was determined by Western blot using a phospho-Tau antibody.
Biological activity:
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 53.0-56.0 kDa
Concentration: N/A
Appearance: Lyophilized protein
Physical form description: Lyophilized from 2 mg/ml in 0.1 M NH4CO3
Reconstitution Instructions: Reconstitute to 1 mg/ml in PBS containing 15% glycerol. The solution should be stored at 70°C, stable for up to 3 months.
Amino acid sequence: N/A
