Product Description
Pyruvate phosphate dikinase, PPDK, (E.C. 2.7.9.1) is found in certain microorganisms and plants. PPDK catalyzes the interconversion of AMP, PPi, and phosphoenolpyruvate (PEP) to ATP, Pi, and pyruvate monophosphate. PPDK plays an important role in the glycolytic pathway and in organisms where pyruvate kinase (PK) is absent. PPDK functions in the process of ATP synthesis. BioVisions active PPDK is suitable for functional assays, high-throughput screening and preclinical studies in drug discovery.
Biovision | P1161 | PDK Active M. rosea Recombinant DataSheet
Biomolecule/Target: PPDK
Synonyms: Pyruvate Phosphate Dikinase, Pyruvate, orthophosphate dikinase
Alternates names: Pyruvate Phosphate Dikinase, Pyruvate, orthophosphate dikinase
Taglines: Plays an important role in the glycolytic pathway
NCBI Gene ID #: 6358
NCBI Gene Symbol: CCL14
Gene Source: Microbispora rosea
Accession #: Q16627
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): Measured by the conversion of 1 mole of AMP, PPi, and phosphoenolpyruvate (PEP) to ATP, Pi and pyruvate monophosphate at 37ºC and pH 7.0.
Biological activity: N/A
Results: 0.8 U/mg
Binding Capacity: N/A
Unit Definition: One unit of enzyme converts 1 mole of AMP, PPi, and phosphoenolpyruvate (PEP) to ATP, Pi and pyruvate monophosphate at 37ºC and pH 7.0.
Molecular Weight: 95.5 kDa
Concentration: N/A
Appearance: Liquid
Physical form description: Proprietary buffer
Reconstitution Instructions: Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 g/ml.). This can further be diluted to other aqueous buffers.
Amino acid sequence: Target protein is expressed with sequence (Thr20-Asn93) of Human hcc-1 (Uniprot #Q16627) fused with a polyhistidine tag at the C-terminus.