Product Description
Protein kinase C delta (PKC delta) is a member of the protein kinase C (PKC) family of serine-threonine kinases. It is a 79 kd protein kinase that shows strict dependence on the presence of phospholipids, but shows no activation by Ca2+ (1). Phosphatidylinositol is the most potent activator of PKC delta. Apparent kinetic constants for synthetic oligopeptides (MBP4-14, EGFR peptide and epsilon-peptide) are quite different between PKC delta and other PKCs. Northern blot analysis indicated that PKC delta is widely distributed in almost all the tissues and is a major isoform of PKC expressed in hemopoietic cells (2). PKC delta is involved in fundamental cellular functions regulated by diacylglycerols and mimicked by phorbol esters. PKC delta is partially associated with the insoluble fraction in cells even in the absence of phorbol 12-myristate 13-acetate (PMA). Upon PMA stimulation, both it translocate to the insoluble fraction of cell homogenates (3). Overexpression of PKC-delta induces significant changes in morphology and causes the cells to grow more slowly and to a decreased cell density in confluent cultures. These changes are accentuated by treatment with PMA. None of the PKC-delta overexpressers grow in soft agar with or without PMA.
Biovision | 7739 | PKC delta Active DataSheet
Biomolecule/Target: N/A
Synonyms: PKC, Protein kinase C delta
Alternates names: PKC, Protein kinase C delta
Taglines: Phosphorylate a wide variety of protein targets
NCBI Gene ID #: 18049
NCBI Gene Symbol: NGF
Gene Source: N/A
Accession #: P01139
Recombinant: Yes
Source: Baculovirus (Sf9 insect cells)
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): 302 nmol phosphate incorporated into CREBtide substrate per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
Biological activity: This NGF- is fully biologically active when compared to standards. The ED as measured by the dose-dependent induction of human TF-1 cell proliferation is typically 0.11 ng/ml
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 104.0 kDa
Concentration: 5µg/50 µl
Appearance: Liquid
Physical form description: Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml containing 1% HSA or BSA. The solution can be diluted into other aqueous buffers and stored at 4°C for 2-7 days or store at 20°C for future use.
Amino acid sequence: N/A