Product Description
PNP belongs to the PNP/MTAP phosphorylase family of proteins. This protein catalyzes the reversible phosphorolysis of ribonucleosides and 2'-deoxyribonucleosides with specificity for guanine, hypoxanthine and their analogs. PNP deficiency is a rare autosomal recessive genetic disease associated with a severe defect in T-lymphocyte function and neurologic disorder in children, comprising four percent of combined immunodeficiency cases. Recombinant human PNP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques
Biovision | 7831 | PNP human recombinant DataSheet
Biomolecule/Target: PNP
Synonyms: Purine nucleoside phosphorylase, NP, Inosine phosphorylase, PRO1837, PUNP
Alternates names: Purine nucleoside phosphorylase, NP, Inosine phosphorylase, PRO1837, PUNP
Taglines: Catalyzes the reversible phosphorolysis of ribonucleosides and 2'-deoxyribonucleosides
NCBI Gene ID #: 4909
NCBI Gene Symbol: NTF4
Gene Source: Human
Accession #: P34130
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: The ED as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found in the range of 20-50 ng/ml.
Results: Specific activity is > 40,000 pmol/min/µg
Binding Capacity: N/A
Unit Definition: One unit will form 1.0 pmole of 7-Methyl-6-thioguanosine to 7-Methyl-6-thioguanine per minute at PH 7.5 at 25ºC
Molecular Weight: 34.2 kDa (309 aa, 1-289 aa + His Tag)
Concentration: 1 mg/ml
Appearance: Liquid
Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2 mM DTT and 0.1 M NaCl
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or 20°C for future use.
Amino acid sequence: N/A