Product Description
PYCR1 is a universal housekeeping enzyme that catalyzes the NAD (P) H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP (+) in some cell types. It forms a homopolymer and localizes to the mitochondrion. Defects in PYCR1 are the cause of cutis laxa autosomal recessive type 2B (ARCL2B). Recombinant human PYCR1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Biovision | 7368 | PYCR1 human recombinant DataSheet
Biomolecule/Target: PYCR1
Synonyms: Pyrroline-5-carboxylate reductase 1, ARCL2B, P5C, P5CR, PIG45, PP222, PRO3
Alternates names: Pyrroline-5-carboxylate reductase 1, ARCL2B, P5C, P5CR, PIG45, PP222, PRO3
Taglines: A universal housekeeping enzyme that catalyzes the NAD (P) H-dependent conversion of pyrroline-5-carboxylate to proline
NCBI Gene ID #: 81780
NCBI Gene Symbol: CCL5
Gene Source: Human
Accession #: P50231
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 97%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Recombinant Rantes is fully biologically active when compared to standard. The activity is determined by the ability to chemoattract total human lymphocytes and murine T-cells at a concentration between 1-10 ng/ml.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 35.5 kDa (339 aa, 1-319 aa + His Tag).
Concentration: 0.5 mg/ml
Appearance: Liquid
Physical form description: 0.5 mg/ml in 20 mM Tris-HCl buffer (pH 8.5) containing 10% glycerol.
Reconstitution Instructions: It is recommended to reconstitute the lyophilized product with sterile HO at a concentration of 0.1 mgs/ml, which can be further diluted into other aqueous solutions.
Amino acid sequence: N/A