Product Description
QDPR is a member of the short-chain dehydrogenases/reductase (SDR) family of enzymes. Functioning as a homodimer, QDPR plays an important role in the recycling of tetrahydrobiopterin (BH4), an essential cofactor for the hydroxylation of the aromatic amino acids (tryptophan, tyrosine and phenylalanine). More specifically, QDPR catalyzes the regeneration of BH4 from quinonoid dihydrobiopterin (qBH2), the product generated from the hydroxylation reactions. Mutations in the gene encoding QDPR can lead to phenylketonuria II. Recombinant human QDPR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Biovision | 7821 | QDPR human recombinant DataSheet
Biomolecule/Target: QDPR
Synonyms: Quinoid dihydropteridine reductase, DHPR, FLJ42391, PKU2, SDR33C1
Alternates names: Quinoid dihydropteridine reductase, DHPR, FLJ42391, PKU2, SDR33C1
Taglines: Plays an important role in the recycling of tetrahydrobiopterin (BH4)
NCBI Gene ID #: 6288
NCBI Gene Symbol: SAA1
Gene Source: Human
Accession #: P0DJI8
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: For most in vitro applications, apo-SAA exerts its biological activity in the concentration range of 10-50 µg/ml.
Results: Specific activity: > 27 units/ml
Binding Capacity: N/A
Unit Definition: One unit will oxidize 1.0 µmole of b-NADH to b-NAD with 6,7-Dimethyldihydropterine (quinonoid isomer) as the non-nucleotide substrate per minute at pH 7.2 at 25ºC
Molecular Weight: 28.2 kDa (267 aa, 1-244 aa + His Tag)
Concentration: 1 mg/ml
Appearance: Liquid
Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol and 2 mM DTT
Reconstitution Instructions: Reconstitute in 0.1% acetic acid to a concentration of 1 g/l. This solution can then be diluted into other aqueous buffers.
Amino acid sequence: N/A