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Product Description
The R-Spondin (Rspo) proteins belong to the Rspo family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. The Rspo proteins can interact with the Frizzled/LRP6 receptor complex in a manner that causes the stabilization and resulting accumulation of the intracellular signaling protein, -catenin. This activity effectively activates and increases the subsequent nuclear signaling of -catenin. R-Spondin can also bind to the previously discovered G-protein coupled receptors, LGR-4 and LGR-5. Rspo/-catenin signaling can act as an inducer of the transformed phenotype, and can also regulate the proliferation and differentiation of certain stem cell populations. Recombinant human R-Spondin-3 is a 26.9 kDa protein consisting of 240 amino acid residues. Due to glycosylation, R-Spondin-3 migrates at an apparent molecular weight of approximately 37.0 kDa by SDS PAGE analysis under reducing conditions.
Biovision | 7191 | R-Spondin-3 human recombinant DataSheet
Biomolecule/Target: R-Spondin-3
Synonyms: PWTSR, THSD2, Roof plate-specific spondin-3, RSPO3
Alternates names: Fibroblast Growth Factor-17, FGFH
Taglines: Regulates the proliferation and differentiation of certain stem cell populations
NCBI Gene ID #: 8822
NCBI Gene Symbol: FGF17
Gene Source: Human
Accession #: O60258
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): Determined by a cell proliferation assay using balb/c 3T3 cells. The expected ED50 is 10.0 ng/ml, corresponding to a specific activity of 1 x 10^5 units/mg.
Biological activity: Determined by a cell proliferation assay using balb/c 3T3 cells. The expected ED50 is 10.0 ng/ml, corresponding to a specific activity of 1 x 10^5 units/mg.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 22.7 kDa
Concentration: N/A
Appearance: Lyophilized powder
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM sodium phosphate, pH 8.0 and 50 mM NaCl.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Amino acid sequence: MTQGENHPSP NFNQYVRDQG AMTDQLSRRQ IREYQLYSRT SGKHVQVTGR RISATAEDGN KFAKLIVETD TFGSRVRIKG AESEKYICMN KRGKLIGKPS GKSKDCVFTE IVLENNYTAF QNARHEGWFM AFTRQGRPRQ ASRSRQNQRE AHFIKRLYQG QLPFPNHAEK QKQFEFVGSA PTRRTKRTRR PQPLT
