Product Description
West Nile virus (WNV) is a virus of the family Flaviviridae part of the Japanese encephalitis (JE) antigenic complex of viruses. Image reconstructions and cryoelectron microscopy reveal a 45-50 nm virion covered with a relatively smooth protein surface. This structure is similar to virus; both belong to the genus flavivirus within the family Flaviviridae. WNV is a positive-sense, single strand of RNA, it is between 11,000 and 12,000 nucleotides long which encode seven non-structural proteins and three structural proteins. The RNA strand is held within a nucleocapsid formed from 12 kDa protein blocks; the capsid is contained within a host-derived membrane altered by two viral glycoproteins.
Biovision | P1065 | Recombinant West Nile Virus Envelope Protein DataSheet
Biomolecule/Target: N/A
Synonyms: N/A
Alternates names: CCL26
Taglines: N/A
NCBI Gene ID #: 10344
NCBI Gene Symbol: CCL26
Gene Source: Human
Accession #: Q9Y258
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): Determined by its ability to chemoattract human CCR3/HEK 293 cells using a concentration range of 1.0-2.0 g/ml.
Biological activity: Determined by its ability to chemoattract human CCR3/HEK 293 cells using a concentration range of 1.0-2.0 g/ml.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 8.4 kDa
Concentration: N/A
Appearance: Liquid
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Amino acid sequence: TRGSDISKTC CFQYSHKPLP WTWVRSYEFT SNSCSQRAVI FTTKRGKKVC THPRKKWVQK YISLLKTPKQ L