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Product Description
Mouse SF20 is a bone marrow stroma-derived growth factor. SF20 is expressed in the bone marrow, spleen stroma cells, resting mononuclear cells, resting CD8+ and CD19+ cells and activated CD8+ T cells. SF20 has been shown to bind to the surface of cells expressing the receptor TSA-1 (Thymic shared Ag-1). Among SF20s biological activities is stimulation of the proliferation of FDCP2 cells (a mouse factor-dependent hemopoietic cell line) and mouse lymphoid cells. Chromosome 19 Open Reading Frame 10 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 143 amino acids and having a molecular mass of 15.8kDa. The Mouse SF20 is purified by proprietary chromatographic techniques.
Biovision | 4334 | SF20 mouse recombinant DataSheet
Biomolecule/Target: SF20
Synonyms: UPF0556 protein C19orf10 homolog, Stromal cell-derived growth factor SF20, Interleukin-25, IL-25, D17Wsu104e, Il25, SF20, R33729_1, C19orf10, Chromosome 19 Open Reading Frame 10.
Alternates names: Intercellular adhesion molecule 1, major group rhinovirus receptor, CD54 antigen
Taglines: A bone marrow stroma-derived growth factor
NCBI Gene ID #: 3383
NCBI Gene Symbol: ICAM1
Gene Source: Human
Accession #: P05362
Recombinant: Yes
Source: CHO Cells
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): ICAM-1 supported the adhesion of more than 50% PMA-stimulated HSB2 cells to plates coated at 25 µg/ml ICAM-1 concentration.
Biological activity: ICAM-1 supported the adhesion of more than 50% PMA-stimulated HSB2 cells to plates coated at 25 µg/ml ICAM-1 concentration.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 49.5 kDa
Concentration: N/A
Appearance: Lyophilized protein
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.5.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Amino acid sequence: QTSVSPSKVI LPRGGSVLVT CSTSCDQPKL LGIETPLPKK ELLLPGNNRK VYELSNVQED SQPMCYSNCP DGQSTAKTFL TVYWTPERVE LAPLPSWQPV GKNLTLRCQV EGGAPRANLT VVLLRGEKEL KREPAVGEPA EVTTTVLVRR DHHGANFSCR TELDLRPQGL ELFENTSAPY QLQTFVLPAT PPQLVSPRVL EVDTQGTVVC SLDGLFPVSE AQVHLALGDQ RLNPTVTYGN DSFSAKASVS VTAEDEGTQR LTCAVILGNQ SQETLQTVTI YSFPAPNVIL TKPEVSEGTE VTVKCEAHPR AKVTLNGVPA QPLGPRAQLL LKATPEDNGR SFSCSATLEV AGQLIHKNQT RELRVLYGPR LDERDCPGNW TWPENSQQTP MCQAWGNPLP ELKCLKDGTF PLPIGESVTV TRDLEGTYLC RARSTQGEVT RKVTVNVLSP RYE
