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Product Description
Biovision | K2507 | ToxOut™ Phase Extraction Endotoxin Removal Kit DataSheet
Endotoxin is a lipopolysaccharide (LPS) complex located in the outer membrane of gram-negative bacteria. A single E.coli bacterium contains ~2 million LPS molecules. During experimental procedures, large amount of endotoxin is shed and can easily contaminate labware, buffers and downstream products. In vitro, endotoxin causes a variety of problems in cell-based research. In vivo, endotoxin may cause various side effects, including inflammatory response, organ failure or septic shock in host organisms. Therefore, it is critical to remove endotoxin from samples and products. BioVisions ToxOutTM Phase Extraction Endotoxin Removal Kit can effectively eliminate high concentration endotoxin in solutions containing proteins or pharmacologically important components down to <0.05 EU/ml without using toxic buffers, while maintaining protein recovery at >90%. BioVisions ToxOutTM Phase Extraction Endotoxin Removal Kit is based on Triton X-114 Phase Extraction (PE), which shows strong endotoxin removal capabilities. The residual Triton X-114 is further eliminated with our proprietary beads to achieve minimal detergent level in the final samples. The protein recovery is >90% depending on individual protein and typically greater than 95%.
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Tag Line : Quickly and effectively eliminate endotoxins to < 0.05 EU/ml in solutions containing proteins or pharmacologically important components
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Applications : Ideal for processing small scale solution samples (0.1 ml - 0.5 ml),Effectively eliminate endotoxins to < 0.05 EU/ml
Features and Benefits : Final products are suitable for potential downstream therapeutic applications as ligand and buffers are nontoxic,Simple and fast procedure: Takes less than one hour to get endotoxin-free sample,High Capacity: up to 99.99% endotoxin can be removed in a single extraction at 1E+7 EU/ml,One kit can clean up to 5 ml protein sample at 1-5 mg/ml of concentration,Lower endotoxin level to < 0.05 EU/ml in a single extraction from up to 5,000 EU/ml sample,Very low residual detergent: < 1 µg/ml,High Sample Recovery: >90% recovery with protein solution samples (typically > 95%)
