Product Description
Mouse Serine/threonine-protein kinase pim-2 (PIM2) ELISA Kit | KTE70749 | Abbkine
Application: This Mouse Serine/threonine-protein kinase pim-2 (PIM2) ELISA Kit employs a two-site sandwich ELISA to quantitate PIM2 in samples. An antibody specific for PIM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Mouse Serine/threonine-protein kinase pim-2 microplate
• Mouse Serine/threonine-protein kinase pim-2 standard
• Mouse Serine/threonine-protein kinase pim-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Mouse Serine/threonine-protein kinase pim-2 (PIM2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PIM2. No significant cross-reactivity or interference between Mouse PIM2 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: PIM2 encodes a protooncogene that acts as a serine/threonine protein kinase. Studies determined the encoded protein functions to prevent apoptosis and to promote cell survival. The deduced 334-amino acid sequence of the clone showed 90% identity with the mouse Pim2 protein. Like mouse Pim2, the human protein appears to be a serine threonine kinase. Northern blot analysis detected 2 PIM2 transcripts. A 2.2-kb transcript was expressed in all tissues tested, but most abundantly in hematopoietic tissues, spleen, thymus, and peripheral blood leukocytes, as well as in testis, small intestine, and colon. It was also highly expressed in human leukemic and lymphoma cell lines and a colorectal adenocarcinoma cell line. A 5.0-kb transcript was expressed only in spleen, thymus, small intestine, colon, and in very small amounts in peripheral blood leukocytes.
Alternative Names: PIM2; Proto-oncogene Pim-2 (serine threonine kinase) ; serine/threonine protein kinase pim-2
Search name: PIM2; Proto-oncogene Pim-2 (serine threonine kinase) ; serine/threonine protein kinase pim-2
Tag: PIM2