Product Description
Rat Phosphatidylglycerophosphate synthase 1 (PGS1) ELISA Kit | KTE101012 | Abbkine
Application: This Rat Phosphatidylglycerophosphate synthase 1 (PGS1) ELISA Kit employs a two-site sandwich ELISA to quantitate PGS1 in samples. An antibody specific for PGS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Rat Phosphatidylglycerophosphate synthase 1 microplate
• Rat Phosphatidylglycerophosphate synthase 1 standard
• Rat Phosphatidylglycerophosphate synthase 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Rat Phosphatidylglycerophosphate synthase 1 (PGS1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PGS1. No significant cross-reactivity or interference between Rat PGS1 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Phosphatidylglycerophosphate Synthase 1 belongs to the family of transferases, specifically those transferring non-standard substituted phosphate groups. The systematic name of this enzyme class is CDP-diacylglycerol:sn-glycerol-3-phosphate 3-phosphatidyltransferase. This enzyme participates in glycerophospholipid metabolism.Autoradiograms of replica prints, following enzyme assays, showed dark halos indicating the enzymatic synthesis of labeled phospholipid products. The method was also used to detect a cho 1 mutant defective in phosphatidylserine synthase and a strain that overproduces phosphatidylserine synthase. The method should become a valuable tool in isolating yeast strains defective in phospholipid biosynthetic enzyme activities and strains with overproduced enzyme activities.
Alternative Names: PGS1; DKFZp762M186; MGC131960; CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase; mitochondrial; PGP synthase
Search name: PGS1; DKFZp762M186; MGC131960; CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase; mitochondrial; PGP synthase
Tag: PGS1