Product Description
Rat Phosphatidylglycerophosphate synthase 1 (PGS1) ELISA Kit | AE63308RA | Abebio
Species Reactivity: Rat (Rattus norvegicus)
Abbreviation: PGS1
Alternative Name: DKFZp762M186; MGC131960; CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase; mitochondrial|PGP synthase
Application: ELISA
Range: Request Information
Sensitivity: Request Information
Intra-Assay: ≤5.8%
Inter-Assay: ≤8.7%
Recovery: 1, 02
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PGS1 in samples. An antibody specific for PGS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Phosphatidylglycerophosphate Synthase 1 belongs to the family of transferases, specifically those transferring non-standard substituted phosphate groups. The systematic name of this enzyme class is CDP-diacylglycerol:sn-glycerol-3-phosphate 3-phosphatidyltransferase. This enzyme participates in glycerophospholipid metabolism.Autoradiograms of replica prints, following enzyme assays, showed dark halos indicating the enzymatic synthesis of labeled phospholipid products. The method was also used to detect a cho 1 mutant defective in phosphatidylserine synthase and a strain that overproduces phosphatidylserine synthase. The method should become a valuable tool in isolating yeast strains defective in phospholipid biosynthetic enzyme activities and strains with overproduced enzyme activities.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .