Product Description
mAB-O | Mouse anti-human Abeta protein (3-10) region, oligomer-specific (clone 3E5, F8) | AS13 2715 | Agrisera
Immunogen: Synthetic peptide chosen from human Abeta (1-42) protein,
Host: Mouse
Conjugation: N/A
Clonality: Monoclonal
Isotype: IgG1, kappa light chain, (clone number 3E5, F8)
Purity: Immunogen affinity purified serum in PBS pH 7.4.
Format: Lyophilized
Tested Application: Dot blot (Dot), ELISA (ELISA), Immunolocalization (IL)
Related Products: AS10 932 | Amyloid beta oligomer-specific monoclonal antibody (OMAB) AS13 2716 | mAB-M | human Abeta (3-10) region, monoclonal antibody
Recommended Dilutions: 10 ug/ml (IL), 1-2 ug/ml (Dot), 1 ug/ml (ELISA capture)
Molecular weight: 4, 5 kDa
Confirmed Reactivity: Human
Predicted Reactivity: Mouse, Bovine, Chicken, Dog, Porcine, Rabbit
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: Immunolocalization: human tissue was paraffin-embedded and sectioned. De-waxed and rehydrated in an ethanol gradient. Antigens were retrieved in sodium citrate buffer (pH 6) at 95°C for 1 h. The tissue sections were separately incubated for 1 h at RT with primary antibody and antibody binding was visualized with IgG Preoxidase Reagent Kit.This Monoclonal IgG1, kappa light chain, (clone number 3E5.F8) is specific for human Amyloid-Beta oligomers.
Background: Alzheimer's disease (AD) is the most prevalent neurodegenerative disease in the growing population of elderly people. A hallmark of AD is the accumulation of plaques in the brain of AD patients. The plaques predominantly consist of aggregates of amyloid-beta (Abeta), a peptide of 39-42 amino acids generated in vivo by specific, proteolytic cleavage of the amyloid precursor protein. Recent findings however suggest that smaller oligomeric forms of Abeta, formed in parallel to the amyloid plaques, excert the predominant tissue damaging effect.Specific identification of the oligomeric forms is as a consequence of great interest. Based on a recently published technique a highly oligomer-specific antibody (mAB-O), targeting Abeta oligomers while omitting reactivity towards the monomeric and fibrillar counterpart, has been developed.
Reconstitution: For reconstitution add 50 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: N/A
Category: Alzheimer's disease
Research Area: Pathlogy, Alzheimer's disease