Product Description
Proteins expressed in the endoplasmic reticulum (ER) are tightly regulated by a variety of quality control mechanisms. Terminally misfolded proteins in the ER are retrotranslocated to the cytoplasm and degraded by proteasomes through a mechanism known as ER-associated degradation (ERAD). EDEM2 (ER degradation-enhancing alpha-mannosidase-like 2) is a 578 amino acid secreted protein that, in conjunction with other EDEM proteins (namely EDEM and EDEM3), is involved in the ERAD pathway of protein degradation. EDEM2, a member of the glycosyl hydrolase 47 family, contains a mannosidase homology domain, an N-terminal cleavable signal sequence and a C-terminal extension that is required for both ER retention and the proper function of EDEM2. Human EDEM2 shares 93% sequence identity with its mouse homolog, suggesting a conserved role between species. Two isoforms of EDEM2 exist due to alternative splicing events.
Biovision | 6633 | EDEM2 Antibody DataSheet
Antibody Target: EDEM2
Target Alternative Name: ER degradation-enhancing alpha-mannosidase-like protein 2
Tag Line: Polyclonal antibody to detect EDEM2 in human, mouse and rat samples
Category: EDEM2
Host: Rabbit
Isotype: Rabbit IgG
Species Reactivities: Human, mouse, rat
Immunogen Sequence: Synthetic peptide C-terminal
Accession #: Q9BV94
Gene ID: 55741
Appearance: Colorless liquid
Form: Liquid
Concentration: 0.5 mg/ml
Formulation: 30 µg (0.5 mg/ml) polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Purification: Affinity purified antibody
Application: Western blot
Positive Control: Western Blot: Rat kidney lysate
Application And Usages: Western blot: 1:200
Country of Animal Origin: USA
Country of Manufacture: USA
Usage: For Research Use Only! Not to be used in humans.
Handling: The antibody solution should be gently mixed before use.
Western Blot Verified: Verified
Immunocytochemistry Verified: Not Verified
Immunofluorescence Verified: Not Verified
Immunoprecipitation Verified: Not Verified
FACS Verified:
ELISA Verified: Not Verified
ChIP Verified: Not Verified
Dot Blot Verified:
Flow Cytometry Verified: Not Verified