Product Description
Alkaline Phosphatase (ALP) is a hydrolase enzyme responsible for removing phosphate groups in the 5- and 3- positions from many types of molecules, including nucleotides, proteins, and alkaloids. In humans, alkaline phosphatase is present in all tissues throughout the entire body, but is particularly concentrated in liver, bile duct, kidney, bone, and the placenta. The optimal pH for the enzyme activity is pH 10 in standard conditions. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.
Antibody Target: PLAP (Placental alkaline phosphatase)
Target Alternative Name:
Tag Line: affinity purified Rabbit monoclonal to PLAP
Category: Primary antibody
Host: Rabbit
Isotype: Rabbit IgG
Species Reactivities: Human
Immunogen Sequence: A peptide corresponding to the N-terminus of human PLAP (Placental alkaline phosphatase)
Accession #: P05187
Gene ID: 250
Appearance: Liquid
Form: Liquid
Concentration:
Formulation: 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Purification: Protein A affinity purified from an animal originfree culture supernatant
Application: IHC: 1:500-1:1000, Western Blot: 1:1000-1:2000
Positive Control:
Application And Usages: IHC: 1:500-1:1000, Western Blot: 1:1000-1:2000
Country of Animal Origin: USA
Country of Manufacture: USA
Usage: For Research Use Only! Not to be used in humans.
Handling: The antibody solution should be gently mixed before use.
Western Blot Verified: True
Immunocytochemistry Verified: True
Immunofluorescence Verified: False
Immunoprecipitation Verified: False
FACS Verified: False
ELISA Verified: False
ChIP Verified: False
Dot Blot Verified: False
Flow Cytometry Verified: False