Product Description
ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated. Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Severing activity is not dependent on tubulin acetylation or detyrosination. Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. It is critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. SPAST is involved in abscission step of cytokinesis and nuclear envelope reassembly during anaphase in cooperation with the ESCRT-III complex.
Biovision | A1855 | Anti-Spastin Rabbit Monoclonal Antibody (RM346) DataSheet
Antibody Target: Spastin
Target Alternative Name:
Tag Line: Affinity purified Rabbit monoclonal to Spastin
Category: Primary antibody
Host: Rabbit
Isotype: Rabbit IgG
Species Reactivities: Human
Immunogen Sequence: A peptide corresponding to the N-terminus of human Spastin.
Accession #: Q9UBP0
Gene ID: 6683
Appearance: Liquid
Form: Liquid
Concentration:
Formulation: 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Purification: Protein A affinity purified from an animal originfree culture supernatant
Application: Western Blot: 1:500-1:1000, IHC: 1:500-1:1000
Positive Control:
Application And Usages: Western Blot: 1:500-1:1000, IHC: 1:500-1:1000
Country of Animal Origin: USA
Country of Manufacture: USA
Usage: For Research Use Only! Not to be used in humans.
Handling: The antibody solution should be gently mixed before use.
Western Blot Verified: True
Immunocytochemistry Verified: True
Immunofluorescence Verified: False
Immunoprecipitation Verified: False
FACS Verified: False
ELISA Verified: False
ChIP Verified: False
Dot Blot Verified: False
Flow Cytometry Verified: False