Product Description
A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It is useful for a variety of applications such as isolation of DNA and RNA, preparation of chromosomal DNA, etc. It retains its activity in the presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF).
Biovision | 9252 | EZSolution™ Proteinase K DataSheet
Biomolecule/Target :
Synonyms: Protease K, Endopeptidase K, Tritirachium alkaline proteinase
Alternates names: Protease K, Endopeptidase K, Tritirachium alkaline proteinase
Taglines: A serine protease which inactivates nucleases
NCBI Gene ID #: 5621
NCBI Gene Symbol:
Gene Source: N/A
Accession #: P06873
Recombinant: True
Source: Tritirachium album
Purity by SDS-PAGE: ≥ 99% by SDS-PAGE (Native PAGE and SDS PAGE assay)
Assay:
Purity:
Assay #2:
Endotoxin Level:
Activity (Specifications/test method): ≥ 30 units/mg
Biological activity:
Results:
Binding Capacity:
Unit Definition: One unit is defined as the amount of enzyme that will liberate 1 umol of tyrosine per minute at 37 °C, pH 7.5.
Molecular Weight: 28.9 kDa
Concentration:
Appearance: Liquid
Physical form description:
Reconstitution Instructions:
Amino acid sequence:
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans