Product Description
PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Biovision | P1197 | Cyclophilin B Mouse Recombinant DataSheet
Biomolecule/Target: Ppib
Synonyms: AA408962, AA553318, AI844835, Cphn-2, Cphn2, CyP-20b
Alternates names: OB Protein, Obesity Protein, OBS, Obesity factor, HuB219, OB receptor, CD_antigen=CD295
Taglines: An immunophilin family protein catalyzing the isomerization of peptide bonds
NCBI Gene ID #: 3952
NCBI Gene Symbol: LEP
Gene Source: Mouse
Accession #: P48357
Recombinant: Yes
Source: E.coli
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): Measured by its ability to cleaves 1 nmole of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
Biological activity: N/A
Results: >1,000 nmol/min/mg
Binding Capacity: N/A
Unit Definition: One unit is defined as the amount of enzyme that cleaves 1 nmole of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
Molecular Weight: N/A
Concentration: N/A
Appearance: Liquid
Physical form description: In Phosphate Buffered Saline (pH7.4) containing 10% glycerol
Reconstitution Instructions: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 g/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles
Amino acid sequence: Phe22-Asp839