Product Description
Fructose-1, 6-bisphosphatase 1(FBP1) is a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1, 6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1, 6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis. Recombinant FBP1 protein, fused to His-tag at N-terminus, was expressed in E.Coli and purified by using conventional chromatography techniques.
Biovision | 7803 | FBP1 human recombinant DataSheet
Biomolecule/Target: FBP1
Synonyms: Fructose-1,6-bisphosphatase 1, FBPase1, FBP, FBP1
Alternates names: Fructose-1,6-bisphosphatase 1, FBPase1, FBP, FBP1
Taglines: A gluconeogenesis regulatory enzyme
NCBI Gene ID #: 54250
NCBI Gene Symbol: FGF-2
Gene Source: Human
Accession #: P13109
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 97%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Specific activity is > 0.30 unit/ml, in which one unit will convert 1.0 µmole of fructose 1, 6-diphosphate to fructose 6-phosphate and inorganic phosphate per minute at pH 9.5 at 25ºC
Results: Specific activity is > 0.30 unit/ml
Binding Capacity: N/A
Unit Definition: One unit will convert 1.0 µmole of fructose 1, 6-diphosphate to fructose 6-phosphate and inorganic phosphate per minute at pH 9.5 at 25ºC
Molecular Weight: 39.0 kDa (358 aa, 1-338 aa + His Tag)
Concentration: 1 mg/ml
Appearance: Liquid
Physical form description: 1 mg/ml solution 20 mM Tris-HCl buffer, pH 8.0, 10% glycerol and 1 mM DTT.
Reconstitution Instructions: N/A
Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MADQAPFDTD VNTLTRFVME EGRKARGTGE LTQLLNSLCT AVKAISSAVR KAGIAHLYGI AGSTNVTGDQ VKKLDVLSND LVMNMLKSSF ATCVLVSEED KHAIIVEPEK RGKYVVCFDP LDGSSNIDCL VSVGTIFGIY RKKSTDEPSE KDALQPGRNL VAAGYALYGS ATMLVLAMDC GVNCFMLDPA IGEFILVDKD VKIKKKGKIY SLNEGYARDF DPAVTEYIQR KKFPPDNSAP YGARYVGSMV ADVHRTLVYG GIFLYPANKK SPNGKLRLLY ECNPMAYVME KAGGMATTGK EAVLDVIPTD IHQRAPVILG SPDDVLEFLK VYEKHSAQ